期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 319, 期 1-2, 页码 41-52出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2006.10.008
关键词
human; T cells; cell surface molecules; transcription factors
资金
- NIAID NIH HHS [R37 AI40312, F32 AI067088] Funding Source: Medline
- NIH HHS [OD00329] Funding Source: Medline
Although quantitative identification and viable enrichment of natural regulatory T cells (T-regs) in humans are problematic, such steps would greatly facilitate the analysis of these cells in disease states. In an attempt to identify markers that are sensitive and specific for human T-regs, we analyzed the expression of fourteen intracellular and cell surface markers on human CD4(+) cells. Many markers were partially selective for CD251(hi) T-regs, but consistent and specific discrimination of functional T-regs was only made possible by focus on CD 127, the alpha chain of the IL-7 receptor. Although most CD4(+) human T cells express CD 127, T-regs exhibiting suppressive activity in vitro display distinctly lower surface expression of this marker, irrespective of their level of CD25 expression. Soiled cells with the surface phenotype CD4(+)CD25(+)CDI27(low) had higher levels of intracellular FOXP3 and CTLA-4 and, as determined by functional assays, were suppressive, hypoproliferative, and poorly responsive to TCR signaling. The CD4(+)CD25(+)CD127(low) phenotype was also found to be characteristic of T-regs found in once and in rhesus macaques. This surface phenotype should allow for quantitative studies of regulatory T cells in disease states as well as for enrichment of live regulatory T cells for functional analyses and/or expansion in vitro. (c) 2006 Published by Elsevier B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据