期刊
BIOCHIMIE
卷 89, 期 2, 页码 222-229出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.biochi.2006.09.013
关键词
Arabidopsis thaliana; oil bodies; short-chain dehydrogenase reductase; hydroxysteroid dehydrogenase reductase; estradiol oxidation
In a previous work, we presented evidence for the presence of a protein encoded by At5g50600 in oil bodies (OBs) from Arabidopsis thaliana [P. Jolivet, E. Roux, S. D'Andrea, M. Davanture, L. Negroni, M. Zivy, T. Charclot, Protein composition of oil bodies in Arabidopsis thaliana lecotype WS, Plant Physiol. Biochem. 42 (2004) 501-509]. Using specific antibodies and proteomi c techniques, we presently confirm the existence of this protein, which is a member of the short-chain steroid dehydrogenase reductase superfamily. We have measured its activity toward various steroids (cholesterol, dehydroepiandrosterone, cortisol, corticosterone, estradiol, estrone) and NAD(P)(H), either within purified OBs or as a purified bacterially expressed chimera. Both enzymatic systems (OBs purified from A. thaliana seeds as well as the chimeric enzyme) exhibited hydroxysteroid dehydrogenase (HSD) activity toward estradiol (17 beta-hydroxysteroid) with NAD+ or NADP(+) NADP+ being the preferred cofactor. Low levels of activity were observed with cortisol or corticosterone (Ill3-hydroxysteroids), but neither cholesterol nor DHEA (3 beta-hydroxysteroids) were substrates, whatever the cofactor used. Similar activity profiles were found for both enzyme sources. Purified OBs were found to be also able to catalyze estrone reduction (17 beta-ketosteroid reductase activity) with NADPH. The enzyme occurring in A. thaliana OBs can be classified as a NADP+-dependent 11 beta,17 beta-hydroxysteroid dehydrogenase/17 beta-ketosteroid reductase. This enzyme probably corresponds to AtHSD1, which is encoded by At5g50600. However, its physiological role and substrates still remain to be determined. (c) 2006 Elsevier Masson SAS. All rights reserved.
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