期刊
PLOS PATHOGENS
卷 3, 期 2, 页码 200-211出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.ppat.0030014
关键词
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资金
- Intramural NIH HHS [Z01 AI005093] Funding Source: Medline
- NIAID NIH HHS [AI05093, 1R01AI045806-01A1, R01 AI045806] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
Toxoplasma gondii is an obligate intracellular parasite for which the discharge of apical organelles named rhoptries is a key event in host cell invasion. Among rhoptry proteins, ROP2, which is the prototype of a large protein family, is translocated in the parasitophorous vacuole membrane during invasion. The ROP2 family members are related to protein-kinases, but only some of them are predicted to be catalytically active, and none of the latter has been characterized so far. We show here that ROP18, a member of the ROP2 family, is located in the rhoptries and re-localises at the parasitophorous vacuole membrane during invasion. We demonstrate that a recombinant ROP18 catalytic domain (amino acids 243-539) possesses a protein-kinase activity and phosphorylate parasitic substrates, especially a 70-kDa protein of tachyzoites. Furthermore, we show that overexpression of ROP18 in transgenic parasites causes a dramatic increase in intra-vacuolar parasite multiplication rate, which is correlated with kinase activity. Therefore, we demonstrate, to our knowledge for the first time, that rhoptries can discharge active protein-kinases upon host cell invasion, which can exert a long-lasting effect on intracellular parasite development and virulence.
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