期刊
MICROSCOPY RESEARCH AND TECHNIQUE
卷 70, 期 2, 页码 154-161出版社
WILEY-LISS
DOI: 10.1002/jemt.20395
关键词
two-photon microscopy; multiphoton tomography; hair; melanin fluorescence; FLIM; time resolved single-photon counting; eumelanin; pheomelanin
In vivo and in vitro multiphoton imaging was used to perform high resolution optical sectioning of human hair by nonlinear excitation of endogenous as well as exogenous fluorophores. Multiphoton fluorescence lifetime imaging (FLIM) based on time-resolved single photon counting and near-infrared femtosecond laser pulse excitation was employed to analyze the various fluorescent hair components. Time-resolved multiphoton imaging of intratissue pigments has the potential (i) to identify endogenous keratin and melanin, (ii) to obtain information on intrahair dye accumulation, (iii) to study bleaching effects, and (iv) to monitor the intratissue diffusion of pharmaceutical and cosmetical components along hair shafts.
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