Effects of frozen storage temperature on the elasticity of tendons from a small murine model

The basic mechanism of reinforcement in tendons addresses the transfer of stress, generated by the deforming proteoglycan (PG)rich matrix, to the collagen fibrils. Regulating this mechanism involves the interactions of PGs on the fibril with those in the surrounding matrix and between PGs on adjacent fibrils. This understanding is key to establishing new insights on the biomechanics of tendon in various research domains. However, the experimental designs in many studies often involved long sample preparation time. To minimise biological degradation the tendons are usually stored by freezing. Here, we have investigated the effects of commonly used frozen storage temperatures on the mechanical properties of tendons from the tail of a murine model (C57BL6 mouse). Fresh (unfrozen) and thawed samples, frozen at temperatures of -20 degrees C and -80 degrees C, respectively, were stretched to rupture. Freezing at -20 degrees C revealed no effect on the maximum stress (sigma, stiffness (E), the corresponding strain (epsilon) at sigma and strain energy densities up to epsilon(u) and from epsilon until complete rupture (u(p)). On the other hand, freezing at -80 degrees C led to higher sigma, E and u; epsilon and u(p) were unaffected. The results implicate changes in the long-range order of radially packed collagen molecules in fibrils, resulting in fibril rupture at higher stresses, and changes to the composition of extra fibrillar matrix, resulting in an increase in the interaction energy between fibrils via collagen-bound PGs.