期刊
GENE THERAPY
卷 14, 期 3, 页码 275-280出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.gt.3302867
关键词
electroporation; skin; electrode design
类别
资金
- NIBIB NIH HHS [R01 EB005441, R01 EB005441-01A2] Funding Source: Medline
- NIDDK NIH HHS [R21 DK055588-02, R21 DK055588-01A1, R21 DK55588] Funding Source: Medline
The easy accessibility of skin makes it an excellent target for gene transfer protocols. To take advantage of skin as a target for gene transfer, it is important to establish an efficient and reproducible delivery system. Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo. A critical component of this technique is the electrode configuration. Electroporation parameters were optimized for transgene expression with minimal tissue damage with a novel electrode. The highest transgene expression and efficiency of individual cell transformation with minimal damage was produced with eight 150 ms pulses at field strength of 100 V/cm. This electrode design offers the potential for easier and more reproducible electrically mediated cutaneous plasmid delivery than the simple electrodes currently commercially available. This electrode can be a valuable tool in determining the applicability of electrically mediated cutaneous gene transfer.
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