期刊
JOURNAL OF MICROSCOPY
卷 225, 期 2, 页码 175-182出版社
WILEY
DOI: 10.1111/j.1365-2818.2007.01729.x
关键词
coherent anti-Stokes Raman scattering; in vivo imaging; myelin
类别
资金
- NIBIB NIH HHS [R01 EB007243-01, R01 EB007243, R21 EB004966-02, R21 EB004966, R21 EB004966-01] Funding Source: Medline
We report in vivo nonlinear optical imaging of mouse sciatic nerve tissue by epidetected coherent anti-Stokes Raman scattering and second harmonic generation microscopy. Following a minimally invasive surgery to open the skin, coherent anti-Stokes Raman scattering imaging of myelinated axons and second harmonic generation imaging of the surrounding collagen fibres were demonstrated with high signal-to-background ratio, three-dimensional spatial resolution, and no need for labelling. The underlying contrast mechanisms of in vivo coherent anti-Stokes Raman scattering were explored by three-dimensional imaging of fat cells that surround the nerve. The epidetected coherent anti-Stokes Raman scattering signals from the nerve tissues were found to arise from interfaces as well as back reflection of forward coherent anti-Stokes Raman scattering.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据