期刊
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
卷 29, 期 2, 页码 159-164出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2006.09.005
关键词
CTX-M-14; extended-spectrum beta-lactamase; antibiotic resistance; kinetic study
Cefotaxime-resistant Escherichia coli TUM 1121 was isolated from an abscess of an 83-year-old patient. The CTX-M-14 gene was located on a 70 kb plasmid. The enzyme was purified and its activity was analysed. CTX-M-14 was poorly active against ceftazidime and aztreonam. Aztreonam behaved as a competitive inhibitor. Among the tested suicide substrates for class A beta-lactamases, sulbactam was a rather good substrate. Tazobactam and clavulanic acid behaved as inactivators. The interactions between clavulanic acid and CTX-M-14 were characterised by progressive inactivation of the P-lactamase. Carbapenems such as imipenem, meropenem or doripenem did not behave as inactivators of CTX-M-14, however very small k(cat) values were observed. This result shows that CTX-M-14 is able to hydrolyse carbapenems. (c) 2006 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
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