期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 96, 期 2, 页码 318-325出版社
WILEY
DOI: 10.1002/bit.21092
关键词
fluorescent nanosensors; oxygen detection; self-assembly on cell surfaces
We describe a strategy for the preparation and self-assembly of fluorescent nanosensors onto Saccharomyces cerevisiae cell surfaces for dynamically measuring oxygen concentration in the proximity of living cells. Amine functionalized polystyrene nanobeads were impregnated with an oxygen-sensitive ruthenium (II) complex and the beads' surface was coated with polyethylenimine. The resulting nanosensors were assembled on individual S. cerevisiae cells in a controlled manner at physiological pH for continuously monitoring oxygen consumption. This approach exemplifies a general scheme for assembling fluorescent nanosensors on cells for the non-invasive, reversible, and real-time measurement of other physiologically relevant processes, such as the efflux of protons and carbon dioxide, of the influx of glucose.
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