期刊
BIOTECHNOLOGY LETTERS
卷 29, 期 2, 页码 291-294出版社
SPRINGER
DOI: 10.1007/s10529-006-9230-4
关键词
calcium; Candida magnoliae; copper; mannitol
Supplementation with CaCl2 center dot 2H(2)O (50 mg l(-1)) or CuSO4 center dot 5H(2)O (10 mg l(-1)) improved mannitol production by Candida magnoliae by 14.5 and 18.6% (25 and 32 g/L), respectively. When used in combination, they acted synergistically: Ca2+ decreased the intracellular concentration of mannitol 30%, whereas Cu2+ increased the intracellular activity of mannitol dehydrogenase 1.6-times more than control. Ca2+ probably works by altering the permeability of cells to mannitol, whereas, Cu2+ increases the activity of an enzyme responsible for mannitol biosynthesis.
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