期刊
BIORESOURCE TECHNOLOGY
卷 98, 期 3, 页码 504-510出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2006.02.009
关键词
solid-state fermentation; Box-Behnken designs; multiplicity; isoforms; protease; post-translational modifications
This study reports the production of xylanolytic and cellulolytic enzymes by a thermophilic fungal isolate Myceliophthora sp. using a cheap medium containing rice straw and chemically defined basal medium under solid-state culture. A combination of one factor at a time approach followed by response surface methodology using Box-Behnken design of experiments resulted in 2.5, 1.25, 1.28 and 4.23 fold increase in xylanase, endoglucanase, beta-glucosidase and FPase activity, respectively. The zymograms developed against IEF gels showed that multiple isoforms of xylanase (5), endoglucanase (4) and beta-glucosidase (2) were produced under optimized culture conditions. Moreover, thiol containing serine proteases produced during the growth of the culture had no role in the post-translational modification of these xylanases. (c) 2006 Elsevier Ltd. All rights reserved.
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