期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 292, 期 2, 页码 H1042-H1050出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00800.2006
关键词
oxidative stress; cerebrovasculature; myogenic activity; actin cytoskeleton
资金
- NINDS NIH HHS [R01-NS-043316, R01 NS040071, R01-NS-045940, R01 NS043316, R01-NS-040071] Funding Source: Medline
This study investigated the effect of peroxynitrite (OONO-)-induced nitrosylation of filamentous (F)-actin on myogenic tone in isolated and pressurized posterior cerebral arteries (PCAs). Immunohistochemical staining was used to determine 3-nitrotyrosine (NT) and F-actin content in vascular smooth muscle after exposure to 10(-7) M or 10(-4) M OONO- for 5 or 60 min in isolated third-order PCAs (n = 37) from male Wistar rats pressurized to 75 mmHg in an arteriograph chamber, quantified with confocal microscopy. Additionally, the role of K+ channels in OONO--induced dilation was investigated with 3 mu M glibenclamide or 10 mM tetraethylammonium chloride before OONO- exposure. OONO- (10(-4) M) induced a 40% dilation of tone (P < 0.05) while diminishing F-actin content by half (P < 0.05) and causing a 60-fold increase in NT (P < 0.05) in the vascular smooth muscle of PCAs. Additionally, F-actin was inversely correlated with both diameter and NT content (P < 0.05) and was significantly colocalized in the vascular smooth muscle with NT (overlap coefficient = 0.8). The dilation to ONOO- was independent of K+ channel activity and thiol oxidation as glibenclamide, tetraethylammonium chloride, and dithiothreitol had no effect on OONO--induced dilation or F-actin or NT content in PCAs. Because NT was colocalized with F-actin, we hypothesize that OONO- induces nitrosylation of F-actin in vascular smooth muscle leading to depolymerization and the subsequent loss of myogenic tone, which may promote vascular damage during oxidative stress such as in ischemia and reperfusion injury.
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