期刊
MOLECULAR AND CELLULAR BIOCHEMISTRY
卷 296, 期 1-2, 页码 121-127出版社
SPRINGER
DOI: 10.1007/s11010-006-9305-5
关键词
cardiac metabolism; fatty acid oxidation; lactate oxidation
类别
The goal of this study was to investigate the effect of 1 mM exogenous lactate on cardiac function, and some metabolic parameters, such as glycolysis, glucose oxidation, lactate oxidation, and fatty acid oxidation, in isolated working rat hearts. Hearts from male Sprague-Dawley rats were isolated and perfused with 5 mM glucose, 1.2 mM palmitate, and 100 mu U/ml insulin with or without 1 mM lactate. The rates of glycolysis, glucose, lactate, and fatty acid oxidation were determined by supplementing the buffer with radiolabeled substrates. Cardiac function was similar between lactate+ and lactate- hearts. Glycolysis was not affected by 1 mM lactate. The addition of lactate did not alter glucose oxidation rates. Interestingly, palmitate oxidation rates almost doubled when 1 mM lactate was present in the perfusate. This study suggests that substrate supply to the heart is crucially important when evaluating the data from metabolic studies.
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