Flavobacterium defluvii sp nov., isolated from activated sludge

A Gram-negative bacterium, designated strain EMB117(T), was isolated from a municipal wastewater treatment plant and characterized by polyphasic taxonomy. The cells were non-spore-forming rods that showed gliding motility. Optimal growth occurred at 25-30 degrees C and pH 7.0-8.0. Strain EMB117(T) contained phosphaticlylethanolamine as the predominant polar lipid, and the major fatty acids were iso-C-15:0, iSO-Cl-17:0 3-OH, iSO-C-15:0 3-OH and summed feature 3 (C-16: 1 omega 7c and/or iSO-Cl-15:0 2-OH). The G + C content of the genomic DNA was 33.5 mol% and the major isoprenoid quinone was MK-6. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain EMB1 17 T belonged to the genus Flavobacterium and was most closely related to Flavobacterium johnsoniae DSM 425(T) (97.8% sequence similarity). The DNA-DNA relatedness between strain EMB117(T) and F johnsoniae ATCC 17061(T) was about 18%. On the basis of the phenotypic, chemotaxonomic and molecular data, strain EMB117(T) represents a novel species within the genus Flavobacterium, for which the name Flavobacterium defluvii sp. nov. is proposed. The type strain is EMB117(T) (=KCTC 12612(T) =DSM 17963(T)).