期刊
MOLECULAR BIOTECHNOLOGY
卷 35, 期 2, 页码 141-148出版社
HUMANA PRESS INC
DOI: 10.1007/BF02686109
关键词
Prays oleae; Bacillus thuringiensis; proteolysis; histopathological effect; binding; toxin localization
Considering the fact that Prays oleae is one of the most pathogenic insects to the olive tree in the Mediterranean basin, particularly in Tunisia, the mode of action of Cry insecticidal toxins of Bacillus thuringiensis kurstaki in Prays oleae midgut was investigated. The proteolysis of Bacillus thuringiensis delta-endotoxins in the midgut was a key step in determining their potency against Prays oleae. The latter's proteases activated the delta-endotoxins early, yeilding stable toxins. The in vitro and in vivo binding of these toxins to Prays oleae larvae midgut was studied immunhistochemically, evening a midgut columnar cell vacuolization, microvilli damage, and then a pass of epithelium cell content into the larvae midgut. Moreover, Bacillus thuringiensis toxins were shown to bind to the apical microvilli of the midgut epithelial cells. The in vitro study of the interaction of Prays oleae midgut proteins with biotinylated Bacillus thuringiensis toxins allowed the prediction of four suitable receptor proteins in Prays oleae.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据