期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 57, 期 43, 页码 14075-14079出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201807956
关键词
DNA nanotechnology; DNA-PAINT; microRNA; multiplexing; super-resolution imaging
资金
- National Institutes of Health (NIH) Director's New Innovator Award [1DP2OD007292]
- NIH Transformative Research Award [1R01EB018659]
- NIH grant [5R21HD072481]
- Office of Naval Research (ONR) Young Investigator Program Award [N000141110914]
- ONR grants [N000141612410, N000141010827, N000141310593]
- National Science Foundation (NSF) Faculty Early Career Development Award [CCF1054898]
- NSF grant [CCF1162459]
- Wyss Institute for Biologically Engineering Faculty Startup Fund
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT [R21HD072481] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING [R01EB018659] Funding Source: NIH RePORTER
- OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [DP2OD007292] Funding Source: NIH RePORTER
MicroRNA (miRNA) expression profiles hold promise as biomarkers for diagnostics and prognosis of complex diseases. Herein, we report a super-resolution fluorescence imaging-based digital profiling method for specific, sensitive, and multiplexed detection of miRNAs. In particular, we applied the DNA-PAINT (point accumulation for imaging in nanoscale topography) method to implement a super-resolution geometric barcoding scheme for multiplexed single-molecule miRNA capture and digital counting. Using synthetic DNA nanostructures as a programmable miRNA capture nano-array, we demonstrated high-specificity (single nucleotide mismatch discrimination), multiplexed (8-plex, 2 panels), and sensitive measurements on synthetic miRNA samples, as well as applied one 8-plex panel to measure endogenous miRNAs levels in total RNA extract from HeLa cells.
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