期刊
EMBO REPORTS
卷 8, 期 2, 页码 158-164出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/sj.embor.7400890
关键词
epithelial migration; PALS1-associated tight junction protein; Par3 protein
资金
- NIDDK NIH HHS [P60 DK020572, P60 DK20572, R01 DK058208, DK58208, DK69605, R01 DK069605] Funding Source: Medline
Directional migration is important in wound healing by epithelial cells. Recent studies have shown that polarity proteins such as mammalian Partitioning-defective 6 (Par6), atypical protein kinase C (aPKC) and mammalian Discs large I (Dlg1) are crucial not only for epithelial apico-basal polarity, but also for directional movement. Here, we show that the protein associated with Lin seven 1 (PALS1)-associated tight junction protein (PAT)), another evolutionarily conserved polarity protein, is also required for directional migration by using a wound-induced migration assay. in addition, we found that aPKC and Par3 localize to the leading edge during migration of epithelia and that PAT) regulates their localization. Furthermore, our results show that microtubule-organizing centre orientation is disrupted in PATJ RNA interference (RNAi) MDCKII (Madin-Darby canine kidney 11) cells during migration. Together, our data indicate that PATJ controls directional migration by regulating the localization of aPKC and Par3 to the leading edge. The migration defect in PAT) RNAi cells seems to be due to the disorganization of the microtubule network induced by mislocalization of polarity proteins.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据