期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 34, 页码 8975-8979出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201404766
关键词
nanoparticles; polycations; polymers; protein delivery; structure-function relationships
资金
- National Institutes of Health [CA151884, H55N268201000045C, EB015419]
- David Koch-Prostate Cancer Foundation Program in Cancer Nanotherapeutics, Movember Challenge Award from Prostate Cancer Foundation
- National Research Foundation of Korea [K1A1A2048701]
- NCI [R00A160350]
- PCF Young Investigator Award
- NIH National Research Service Award (NRSA) [1F32CA168163-03]
One limitation of current biodegradable polymeric nanoparticles is their inability to effectively encapsulate and sustainably release proteins while maintaining protein bioactivity. Here we report the engineering of PLGA-polycation nanoparticles with a core shell structure that act as a robust vector for the encapsulation and delivery of proteins and peptides. The optimized nanoparticles can load high amounts of proteins (>20% of nanoparticles by weight) in aqueous solution without organic solvents through electrostatic interactions by simple mixing, thereby forming nanospheres in seconds with diameters <200 nm. The relationship between nanosphere size, surface charge, PLGA-polycation composition, and protein loading is also investigated. The stable nanosphere complexes contain multiple PLGA-polycation nanoparticles, surrounded by large amounts of protein. This study highlights a novel strategy for the delivery of proteins and other relevant molecules.
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