期刊
STEM CELLS
卷 25, 期 2, 页码 411-418出版社
ALPHAMED PRESS
DOI: 10.1634/stemcells.2006-0380
关键词
human embryonic stem cells; dopamine; differentiation; Noggin; Parkinson's disease; 6-hydroxydopamine-lesioned rats
资金
- NINDS NIH HHS [P50 NS-39793, P50 NS039793, P50 NS039793-07] Funding Source: Medline
It is currently not known whether dopamine ( DA) neurons derived from human embryonic stem cells ( hESCs) can survive in vivo and alleviate symptoms in models of Parkinson disease ( PD). Here, we report the use of Noggin ( a bone morphogenic protein antagonist) to induce neuroectodermal cell development and increase the yield of DA neurons from hESCs. A combination of stromal-derived inducing activity and Noggin markedly enhanced the generation of neuroepithelial progenitors that could give rise to DA neurons. In addition, Noggin diminished the occurrence of a fibroblast-like Nestin-positive precursor population that differentiated into myocytes. After transplantation of differentiated hESCs to a rodent model of PD, some grafts contained human midbrain-like DA neurons. This protocol demonstrates hESC derivation and survival of human DA neurons appropriate for cell therapy in PD.
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