期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 41, 页码 10916-10920出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201405742
关键词
dyes/pigments; fluorescent probes; heat shock; imaging agents; lysosomal pH
资金
- NSF of China [21105104, 21275147, 21321003]
- 973 Program [2011CB935800]
- Chinese Academy of Science [KJCX2-EW-N06-01, CMS-PY-201301]
Heat stroke is a life-threatening condition, featuring a high body temperature and malfunction of many organ systems. The relationship between heat shock and lysosomes is poorly understood, mainly because of the lack of a suitable research approach. Herein, by incorporating morpholine into a stable hemicyanine skeleton, we develop a new lysosome-targeting near-infrared ratiometric pH probe. In combination with fluorescence imaging, we show for the first time that the lysosomal pH value increases but never decreases during heat shock, which might result from lysosomal membrane permeabilization. We also demonstrate that this lysosomal pH rise is irreversible in living cells. Moreover, the probe is easy to synthesize, and shows superior overall analytical performance as compared to the existing commercial ones. This enhanced performance may enable it to be widely used in more lysosomal models of living cells and in further revealing the mechanisms underlying heat-related pathology.
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