4.8 Article

Synthesis of and Specific Antibody Generation for Glycopeptides with Arginine N-GlcNAcylation

期刊

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 52, 页码 14517-14521

出版社

WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201407824

关键词

antibodies; arginine; glycopeptides; N-GlcNAcylation; solid-phase peptide synthesis

资金

  1. National Basic Research Program of China (973 program) [2013CB932800]
  2. National Major Project of China [2012ZX09J12108-01, 2012ZX09502-001-005]
  3. NSFC [91313301, 31470245]

向作者/读者索取更多资源

As a unique and unappreciated protein posttranslational modification, arginine N-glycosylation was recently discovered to play an important role in the process that bacteria counteract host defenses. To provide chemical tools for further proteomic and biochemical studies on arginine N-glycosylation, we report the first general strategy for a rapid and cost-effective synthesis of glycopeptides carrying single or multiple arginine N-GlcNAcyl groups. These glycopeptides were successfully utilized to generate the first antibodies that can specifically recognize arginine N-GlcNAcylated peptides or proteins in a sequence-independent manner.

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