期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 25, 页码 6374-6377出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201402037
关键词
cell adhesion; high-throughput screening; peptide arrays; peptides; solid-phase synthesis
资金
- Alberta Glycomics Centre
- University of Alberta Startup Funds
- SENTINEL Bioactive Paper Network
A simple method is described for the patterned deposition of Teflon on paper to create an integrated platform for parallel organic synthesis and cell-based assays. Solvent-repelling barriers made of Teflon-impregnated paper confine organic solvents to specific zones of the patterned array and allow for 96 parallel flow-through syntheses on paper. The confinement and flow-through mixing significantly improves the peptide yield and simplifies the automation of this synthesis. The synthesis of 100 peptides ranging from 7 to 14 amino acids in length gave over 60% purity for the majority of the peptides (> 95% yield per coupling/deprotection cycle). The resulting peptide arrays were used in cell-based screening to identify 14 potent bioactive peptides that support the adhesion or proliferation of breast cancer cells in a 3D environment. In the future, this technology could be used for the screening of more complex phenotypic responses, such as cell migration or differentiation.
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