期刊
BIOTECHNIC & HISTOCHEMISTRY
卷 82, 期 1, 页码 23-28出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10520290701333558
关键词
alcian blue; alizarin red; PCR genotyping
资金
- NICHD NIH HHS [HD22486] Funding Source: Medline
- NIDCR NIH HHS [DE13834] Funding Source: Medline
- NIGMS NIH HHS [GM07413] Funding Source: Medline
Traditionally, cartilage is stained by alcian blue using acidic conditions to differentiate tissue staining. The acidic conditions are problematic when one wishes to stain the same specimen for mineralized bone with alizarin red, because acid demineralizes bone, which negatively affects bone staining. We have developed an acid-free method to stain cartilage and bone simultaneously in zebrafish larvae. This method has the additional advantage that PCR genotyping of stained specimens is possible.
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