期刊
NANOSCALE RESEARCH LETTERS
卷 2, 期 2, 页码 69-74出版社
SPRINGEROPEN
DOI: 10.1007/s11671-006-9032-6
关键词
DNA; compact disc; UV/ozone; hybridization
DNA micropatterns have been fabricated on the surface of Polycarbonate (PC) bases of compact discs, with the ultimate goal of using conventional CD technology for DNA analysis. To confirm the formation of -COOH groups on the PC surface upon UV/ozone treatment, contact angle titrations were carried out on the PC surfaces. The surface morphologies of PC surface were measured by Tapping-mode Atomic Force Microscopy (AFM). As the surface as anchor to attach the amine-modified ssDNA by covalent conjugates via amide bonds, the results of fluorescence spectroscopy showed that the fluorescein labeled complementary ssDNA can be used for detection the ssDNA immobilized on the patterned CD polycarbonate substrate.
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