期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 16, 页码 4107-4112出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201308903
关键词
atomic force microscopy; DNA origami; G-hairpin; G-quadruplex; G-triplex
资金
- Core Research for Evolutional Science and Technology (CREST) of JST
- JSPS KAKENHI [24310097, 24225005, 24104002]
- Mitsubishi Foundation
- Asahi Glass Foundation
- JSPS for the Postdoctoral fellowship
- Grants-in-Aid for Scientific Research [24104002, 11F01345] Funding Source: KAKEN
We present the direct and single-molecule visualization of the in-pathway intermediates of the G-quadruplex folding that have been inaccessible by any experimental method employed to date. Using DNA origami as a novel tool for the structural control and high-speed atomic force microscopy (HS-AFM) for direct visualization, we captured images of the unprecedented solution-state structures of a tetramolecular antiparallel and (3+1)-type G-quadruplex intermediates, such as G-hairpin and G-triplex, with nanometer precision. No such structural information was reported previously with any direct or indirect technique, solution or solid-state, single-molecule or bulk studies, and at any resolution. Based on our results, we proposed a folding mechanism of these G-quadruplexes.
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