期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 30, 页码 7848-7852出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201402980
关键词
combinatorial chemistry; phage display; protein design; RNA binding; zinc fingers
资金
- National Health and Medical Research Council, Australia
The realization that gene transcription is much more pervasive than previously thought and that many diverse RNA species exist in simple as well as complex organisms has triggered efforts to develop functionalized RNA-binding proteins (RBPs) that have the ability to probe and manipulate RNA function. Previously, we showed that the RanBP2-type zinc finger (ZF) domain is a good candidate for an addressable single-stranded-RNA (ssRNA) binding domain that can recognize ssRNA in a modular and specific manner. In the present study, we successfully engineered a sequence specificity change onto this ZF scaffold by using a combinatorial approach based on phage display. This work constitutes a foundation from which a set of RanBP2 ZFs might be developed that is able to recognize any given RNA sequence.
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