期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 36, 页码 9487-9492出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201404099
关键词
cell-surface engineering; electrochemical oxidation; electrochemical reduction; liposomes; smart surfaces
资金
- Carolina Center for Cancer Nanotechnology Excellence (NCI)
- Burroughs Wellcome Foundation
- National Science Foundation
- National Science and Engineering Research Council of Canada (NSERC)
- Canadian Foundation for Innovation (CFI)
We report a strategy to rewire cell surfaces for the dynamic control of ligand composition on cell membranes and the modulation of cell-cell interactions to generate three-dimensional (3D) tissue structures applied to stem-cell differentiation, cell-surface tailoring, and tissue engineering. We tailored cell surfaces with bioorthogonal chemical groups on the basis of a liposome-fusion and -delivery method to create dynamic, electroactive, and switchable cell-tissue assemblies through chemistry involving chemoselective conjugation and release. Each step to modify the cell surface: activation, conjugation, release, and regeneration, can be monitored and modulated by noninvasive, label-free analytical techniques. We demonstrate the utility of this methodology by the conjugation and release of small molecules to and from cell surfaces and by the generation of 3D coculture spheroids and multilayered cell tissues that can be programmed to undergo assembly and disassembly on demand.
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