期刊
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
卷 260, 期 2-3, 页码 137-145出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2006.09.029
关键词
ToF-SIMS; mouse embryo; paraffin-embedded; image PCA
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) equipped with a gold ion gun was used to image mouse embryo sections and differentiate tissue types (brain, spinal cord, skull, rib, heart and liver). Embryos were paraffin-embedded and then deparaffinized. The robustness and repeatability of the method was determined by analyzing ten tissue slices from three different embryos over a period of several weeks. Using principal component analysis (PCA) to reduce the spectral data generated by ToF-SIMS, histopathologically identified tissue types of the mouse embryos can be differentiated based on the characteristic differences in their mass spectra. These results demonstrate the ability of ToF-SIMS to determine subtle chemical differences even in fixed histological specimens. Published by Elsevier B.V.
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