期刊
JOURNAL OF MAGNETIC RESONANCE
卷 184, 期 2, 页码 344-349出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2006.11.002
关键词
in vivo MRS; magnetization transfer; malate dehydrogenase; carbon-13; enzymology
资金
- Intramural NIH HHS [Z01 MH002803-06] Funding Source: Medline
Malate dehydrogenase catalyzes rapid interconversion between dilute metabolites oxaloacetate and malate. Both oxaloacetate and malate are below the detection threshold of in vivo MRS. Oxaloacetate is also in rapid exchange with aspartate catalyzed by aspartate aminotransferase, the latter metabolite is observable in vivo using C-13 MRS. We hypothesized that the rapid turnover of oxaloacetate can effectively relay perturbation of magnetization between malate and aspartate. Here, we report indirect observation of the malate dehydrogenase reaction by saturating malate C2 resonance at 71.2 ppm and detecting a reduced aspartate C2 signal at 53.2 ppm due to relayed magnetization transfer via oxaloacetate C2 at 201.3 ppm. Using this strategy the rate of the cerebral malate clehydrogenase reaction was determined to be 9 +/- 2 mu mol/g wet weight/min (means +/- SD, n = 5) at 11.7 Tesla in anesthetized adult rats infused with [1,6- C-13(2)]glucose. Published by Elsevier Inc.
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