期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 48, 页码 13059-13063出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201405955
关键词
imaging agents; luciferase; luminescence; multiparametric imaging; structure-activity relationships
资金
- BBSRC [BB/I014357/1]
- NIHR UCLH Biomedical Research Centre
- Biotechnology and Biological Sciences Research Council [BB/I014357/1] Funding Source: researchfish
- BBSRC [BB/I014357/1] Funding Source: UKRI
Red-shifted bioluminescent emitters allow improved in vivo tissue penetration and signal quantification, and have led to the development of beetle luciferin analogues that elicit red-shifted bioluminescence with firefly luciferase (Fluc). However, unlike natural luciferin, none have been shown to emit different colors with different luciferases. We have synthesized and tested the first dual-color, far-red to near-infrared (nIR) emitting analogue of beetle luciferin, which, akin to natural luciferin, exhibits pH dependent fluorescence spectra and emits bioluminescence of different colors with different engineered Fluc enzymes. Our analogue produces different far-red to nIR emission maxima up to lambda(max)=706 nm with different Fluc mutants. This emission is the most red-shifted bioluminescence reported without using a resonance energy transfer acceptor. This improvement should allow tissues to be more effectively probed using multiparametric deep-tissue bioluminescence imaging.
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