期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 48, 页码 13110-13114出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201407045
关键词
DNA; G-quadruplexes; genomes; RNA; transcription
资金
- MSTC [2012CB720601, 2013CB530802]
- NSFC [21072189]
G-quadruplexes are implicated in important cellular processes. Previous studies mostly focused on intramolecular G-quadruplexes of three or more G-quartets. Those composed of two G-quartets were only shown to form in single-stranded oligonucleotides. On the basis of electrophoresis, DMS foot-printing, fluorescence labeling, and photo-cross-linking, we detected the formation of DNA: RNA hybrid G-quadruplexes (HQs) of two G-quartets during the transcription of DNA duplexes. These HQs have a lifetime on the minute scale and are stabilized by a stabilizing ligand. They are far shorter-lived than the HQs of three G-quartets, which last for hours. The occurrence of putative formation motifs of such HQs shows a transcription-dependent strand-biased selection, thus supporting their formation and function in genomes. They are present in almost all human genes in large amounts. We speculate that the two-G-quartet HQs may be a distinct type of G-quadruplexes that may play a role in timely responsive processes and for purposes of fine-tuning.
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