期刊
NATURE METHODS
卷 4, 期 2, 页码 123-126出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth995
关键词
-
资金
- NIAID NIH HHS [K24 AI 01744, P30 AI64518, AI 136156, U01 AI062563] Funding Source: Medline
- NIGMS NIH HHS [R01 GM 065057, R01 GM065057] Funding Source: Medline
We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at similar to 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据