期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 21, 页码 5423-5426出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201402623
关键词
antifungal agents; biosynthesis; natural products; polycyclopropanes; polyketides
资金
- MEXT [22108002]
- Grants-in-Aid for Scientific Research [22108002] Funding Source: KAKEN
The biosynthetic gene cluster of antifungal agent jawsamycin (FR-900848) has been identified by heterologous expression. A series of gene inactivations and invitro and invivo analysis of key enzymes in the biosynthetic pathway established their functions. A novel mechanism involving a radical S-adenosyl methionine (SAM) cyclopropanase collaborating with an iterative polyketide synthase is proposed for the construction of the unique polycyclopropanated backbone. Our reconstitution system sets the stage for studying the catalytic mechanism of this intriguing contiguous cyclopropanation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据