期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 24, 页码 6085-6089出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201402030
关键词
acidity; exocytosis; fluorescent probes; spatiotemporal dynamics; vesicular imaging
资金
- SENTAN, JST
- KAKENHI [24750155, 24115502, 25115704, 22000006, 20117003, 23249004, 24116004]
- The Tokyo Society of Medical Sciences
- Research Foundation for Opto-Science and Technology
- Grants-in-Aid for Scientific Research [24116004, 24115502, 25115704, 24590313, 24750155] Funding Source: KAKEN
Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding of secretion phenomena, but current approaches have serious limitations. We designed and synthesized small-molecular fluorescent probes that were chemically optimized for sensing acidic intravesicular pH values, and established that they can be used to sensitively and reliably visualize vesicular dynamics following stimulation. This straightforward technique for the visualization of exocytosis as well as endocytosis/reacidification processes with high spatiotemporal precision is expected to be a powerful tool for investigating dynamic cellular phenomena involving changes in the pH value.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据