期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1141, 期 2, 页码 206-211出版社
ELSEVIER
DOI: 10.1016/j.chroma.2006.12.018
关键词
CE; LIF; red colorants; erythrosine
Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was applied to separation and sensitive determination of red food colorants. Diode pumped frequency-doubled Nd:YAG laser (532 nm) was used as an excitation source in a laboratory-built CE-LIF system. For highly fluorescent erythrosine B (E127), an extrapolated limit of detection (LOD) of 0.4 ng mL(-1) (S/N = 3) was achieved. Extrapolated LODs of other tested red additives, such as carmoisine, E122 (0.5 mu g mL(-1)); amaranth, El 23 (0.2 mu g mL(-1)); ponceau 4R, E124 (0.3 mu g mL(-1)) and red 2G, El 28 (0.3 mu g mL(-1)) were about one-order lower compared to results obtained with CE with absorbance detection in UV/vis (CE-UV/vis). The main advantages of using CE-LIF for analysis of food samples are high selectivity and minimization of matrix effect. To our knowledge, this is the first use of CE-LIF for determination of red food colorants. (c) 2006 Elsevier B.V. All rights reserved.
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