期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 366, 期 1, 页码 244-257出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2006.11.036
关键词
cytoskeleton; adhesion; spectrin repeats; SH3 domain
资金
- NIGMS NIH HHS [R01 GM062270, GM 62270] Funding Source: Medline
- NINDS NIH HHS [NS 47711, R01 NS047711] Funding Source: Medline
Bullous pemphigoid antigen 1 (BPAG1) is a member of the plakin family of proteins. The plakins are multi-domain proteins that have been shown to interact with microtubules, actin filaments and intermediate filaments, as well as proteins found in cellular junctions. These interactions are mediated through different domains on the plakins. The interactions between plakins and components of specialized cell junctions such as desmosomes and hemidesmosomes are mediated through the so-called plakin domain, which is a common feature of the plakins. We report the crystal structure of a stable fragment from BPAG1, residues 226-448, defined by limited proteolysis of the whole plakin domain. The structure, determined by single-wavelength anomalous diffraction phasing from a selenomethionine-substituted crystal at 3.0 angstrom resolution, reveals a tandem pair of triple helical bundles closely related to spectrin repeats. Based on this structure and analysis of sequence conservation, we propose that the architecture of plakin domains is defined by two pairs of spectrin repeats interrupted by a putative Src-Homology 3 (SH3) domain. (c) 2006 Elsevier Ltd. All rights reserved.
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