期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 47, 页码 12729-12734出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201405930
关键词
gels; nonspecific interactions; stem-cell differentiation; zwitterions; zwitterionic hydrogels
资金
- National Science Foundation [DMR 1307375, CBET-1264477]
- Office of Naval Research [N00014-14-1-0090, N00014-09-1-0137]
- National Institutes of Health (NCI T32 grant) [T32A138312]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1264477] Funding Source: National Science Foundation
The success of human mesenchymal stem cell (hMSC) therapies is largely dependent on the ability to maintain the multipotency of cells and control their differentiation. External biochemical and biophysical cues can readily trigger hMSCs to spontaneously differentiate, thus resulting in a rapid decrease in the multipotent cell population and compromising their regenerative capacity. Herein, we demonstrate that nonfouling hydrogels composed of pure poly(carboxybetaine) (PCB) enable hMSCs to retain their stem-cell phenotype and multipotency, independent of differentiation-promoting media, cytoskeletal-manipulation agents, and the stiffness of the hydrogel matrix. Moreover, encapsulated hMSCs can be specifically induced to differentiate down osteogenic or adipogenic pathways by controlling the content of fouling moieties in the PCB hydrogel. This study examines the critical role of nonspecific interactions in stem-cell differentiation and highlights the importance of materials chemistry in maintaining stem-cell multipotency and controlling differentiation.
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