期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 8, 页码 2095-2098出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201309462
关键词
aptamers; molecular imprinting; molecular recognition; responsive hydrogels; viruses
资金
- National Science Foundation [CBET-0854105]
The detection of viruses is of interest for a number of fields including biomedicine, environmental science, and biosecurity. Of particular interest are methods that do not require expensive equipment or trained personnel, especially if the results can be read by the naked eye. A new double imprinting method was developed whereby a virus-bioimprinted hydrogel is further micromolded into a diffraction grating sensor by using imprint-lithography techniques to give a Molecularly Imprinted Polymer Gel Laser Diffraction Sensor (MIP-GLaDiS). A simple laser transmission apparatus was used to measure diffraction, and the system can read by the naked eye to detect the Apple Stem Pitting Virus (ASPV) at concentrations as low as 10ngmL(-1), thus setting the limit of detection of these hydrogels as low as other antigen-binding methods such as ELISA or fluorescence-tag systems.
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