期刊
JOURNAL OF NEUROSCIENCE
卷 27, 期 7, 页码 1616-1630出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3498-06.2007
关键词
perifornical lateral hypothalamus; median preoptic nucleus; electrical stimulation; microdialysis; neuronal activity; sleep-waking cycle
资金
- NHLBI NIH HHS [P50 HL060296, HL60296] Funding Source: Medline
- NIMH NIH HHS [MH63323, R01 MH047480, R01 MH063323, MH47480, R01 MH075076-03, R01 MH075076] Funding Source: Medline
- NINDS NIH HHS [R01 NS050939, NS-50939] Funding Source: Medline
The perifornical-lateral hypothalamic area (PF/LH) contains neuronal groups playing an important role in control of waking and sleep. Among the brain regions that regulate behavioral states, one of the strongest sources of projections to the PF/LH is the median preoptic nucleus (MnPN) containing a sleep-active neuronal population. To evaluate the role of MnPN afferents in the control of PF/LH neuronal activity, we studied the responses of PF/LH cells to electrical stimulation or local chemical manipulation of the MnPN in freely moving rats. Single-pulse electrical stimulation evoked responses in 79% of recorded PF/LH neurons. No cells were activated antidromically. Direct and indirect transynaptic effects depended on sleep-wake discharge pattern of PF/LH cells. The majority of arousal-related neurons, that is, cells discharging at maximal rates during active waking (AW) or during AW and rapid eye movement (REM) sleep, exhibited exclusively or initially inhibitory responses to stimulation. Sleep-related neurons, the cells with elevated discharge during non-REM and REM sleep or selectively active in REM sleep, exhibited exclusively or initially excitatory responses. Activation of the MnPN via microdialytic application of L-glutamate or bicuculline resulted in reduced discharge of arousal-related and in excitation of sleep-related PF/LH neurons. Deactivation of the MnPN with muscimol caused opposite effects. The results indicate that the MnPN contains subset(s) of neurons, which exert inhibitory control over arousal-related and excitatory control over sleep-related PF/LH neurons. We hypothesize that MnPN sleep-active neuronal group has both inhibitory and excitatory outputs that participate in the inhibitory control of arousal-promoting PF/LH mechanisms.
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