期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 19, 页码 4881-4884出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201400531
关键词
cations; hydrogen bonds; intermediates; oligonucleotides; quadruplexes
资金
- Slovenian Research Agency
- Ministry of Higher Education, Science and Technology of the Republic of Slovenia [P1-0242, J1-4020]
- Bio-NMR FP7 project [261863]
- European Cooperation in Science and Technology (COST) [MP0802]
A new folding intermediate of Oxytricha nova telomeric Oxy-1.5 G-quadruplex was characterized in aqueous solution using NMR spectroscopy, native gel electrophoresis, thermal differential spectra (TDS), CD spectroscopy, and differential scanning calorimetry (DSC). NMR experiments have revealed that this intermediate (i-Oxy-1.5) exists in two symmetric bimolecular forms in which all guanine bases are involved in GG N1-carbonyl symmetric base pairs. Kinetic analysis of K+-induced structural transitions shows that folding of Oxy-1.5 G-quadruplex from i-Oxy-1.5 is much faster and proceeds through less intermediates than folding from single strands. Therefore, a new folding pathway of Oxy-1.5 G-quadruplex is proposed. This study provides evidence that G-rich DNA sequences can self-assemble into specific pre-organized DNA structures that are predisposed to fold into G-quadruplex when interacting with cations such as potassium ions.
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