期刊
BIOSENSORS & BIOELECTRONICS
卷 22, 期 7, 页码 1550-1555出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2006.06.018
关键词
G protein-coupled receptors; olfactory receptor 17; self-assembled multilayer; electrochemical impedance spectroscopy; biosensor
In this study, we report a dose-dependent detection of odorant molecules in solution by rat olfactory receptor 17 (OR 17) in its membrane fraction. The OR 17 is immobilized on a gold electrode by multilayer bioengineering based on a mixed self-assembled monolayer and biotin/avidin system, which allows for a well-controlled immobilization of the bioreceptor within its lipid environment. The odorant detection is electronically performed in a quantitative manner by electrochemical impedance spectroscopy (EIS) measurements on samples and controls. circle dot 2006 Elsevier B.V. All rights reserved.
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