期刊
TALANTA
卷 71, 期 2, 页码 537-549出版社
ELSEVIER
DOI: 10.1016/j.talanta.2006.04.027
关键词
arsenic extraction; arsenic speciation; dilute nitric acid; biological tissues
This paper describes the use of dilute nitric acid for the extraction and quantification of arsenic species. A number of extractants (e.g. water, 1.5 M orthophosphoric acid, methanol-water and dilute nitric acid) were tested for the extraction of arsenic from marine biological samples, such as plants that have proved difficult to quantitatively extract. Dilute 2% (v/v) nitric acid was found to give the highest recoveries of arsenic overall and was chosen for further optimisation. The optimal extraction conditions for arsenic were 2% (v/v) HNO3, 6 min(-1), 90 degrees C. Arsenic species were found to be stable under the optimised conditions with the exception of the arsenoriboses which degraded to a product eluting at the same retention time as glycerol arsenoribose. Good agreement was found between the 2% (v/v) HNO3 extraction and the methanol-water extraction for the certified reference material DORM-2 (AB 17.1 and 16.2 mu g g(-1), respectively, and TETRA 0.27 and 0.25 mu g g(-1), respectively), which were in close agreement with the certified concentrations of AB 16.4 +/- 1.1 mu g g(-1) and TETRA 0.248 +/- 0.054 mu g g(-1). To preserve the integrity of arsenic species, a sequential extraction technique was developed where the previously methanol-water extracted pellet was further extracted with 2% (v/v) HNO3 under the optimised conditions. Increases in arsenic recoveries between 13% and 36% were found and speciation of this faction revealed that only inorganic and simple methylated species were extracted. (c) 2006 Elsevier B.V. All rights reserved.
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