期刊
TALANTA
卷 71, 期 2, 页码 976-980出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2006.05.028
关键词
ochratoxin A; stability; microwave-assisted extraction; pressurised liquid extraction; ultrasound-assisted extraction
The stability of Ochratoxin A during its extraction using different extraction techniques has been evaluated. Microwave-assisted extraction and pressurised liquid extraction, in addition to two other reference methods of extraction, i.e. ultrasound-assisted and magnetic stirring-assisted extraction, were evaluated. The effect of extraction temperature using the cited techniques was checked. The results show that Ochratoxin A can be extracted using microwave-assisted extraction at temperatures up to 150 degrees C without degradation. Pressurised liquid extraction can be used at temperatures up to 100 degrees C, for extraction times of less than 30 min. Further, both ultrasound-assisted extraction and magnetic stirring extraction can be applied at temperatures up to 65 degrees C. High-performance liquid chromatography combined with fluorescence detection using a Chromolith RP-18e column at a flow rate of 5 mL min(-1) was used to quantify the Ochratoxin A. The retention time for the Ochratoxin A was 1.3 min. The limits of detection (LOD) and of quantification (LOQ) were 0.03 and 0.10 mu g L-1, respectively. (c) 2006 Elsevier B.V. All rights reserved.
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