期刊
ANALYTICAL CHEMISTRY
卷 79, 期 4, 页码 1604-1610出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac061688m
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资金
- NCRR NIH HHS [R41 RR023764, R41 RR023764-01] Funding Source: Medline
- NHLBI NIH HHS [R01 HL062244, R01 HL062244-07] Funding Source: Medline
- NIGMS NIH HHS [R01 GM038060, R01 GM038060-18, R01 GM038060-19, GM38060] Funding Source: Medline
Polyanionic oligosaccharides such as dermatan sulfate (DS) and chondroitin sulfate (CS) exhibit poor ionization efficiencies and tend to undergo thermal fragmentation through the loss of SO3 under conventional ultraviolet matrix-assisted laser desorption/ionization (UV-MALDI) conditions. A new ionic liquid matrix (ILM), a guanidinium salt of alpha-cyano-4-hydroxycinnamic acid, facilitates direct UV-MALDI mass spectrometric (MS) analysis of underivatized DS and CS oligosaccharides up to a decasaccharide in their common form as sodium salts. The resulting mass spectra show very low extent of fragmentation through an SO3 loss. The new ILM is suitable for MALDI-MS analysis of mixtures containing oligosaccharides with different numbers of sulfo groups.
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