期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 53, 期 35, 页码 9168-9172出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201403580
关键词
bioorthogonal reporters; Diels-Alder cycloaddition; fluorescent probes; metabolic labeling; nucleosides
资金
- Swiss National Science Foundation [146754]
- University of Zurich Forschungskredit [FK-13-113]
5-Vinyl-2'-deoxyuridine (VdU) is the first reported metabolic probe for cellular DNA synthesis that can be visualized by using an inverse electron demand Diels-Alder reaction with a fluorescent tetrazine. VdU is incorporated by endogenous enzymes into the genomes of replicating cells, where it exhibits reduced genotoxicity compared to 5-ethynyl-2'-deoxyuridine (EdU). The VdU-tetrazine ligation reaction is rapid (k approximate to 0.02m(-1) s(-1)) and chemically orthogonal to the alkyne-azide click reaction of EdU-modified DNA. Alkene-tetrazine ligation reactions provide the first alternative to azide-alkyne click reactions for the bioorthogonal chemical labeling of nucleic acids in cells and facilitate time-resolved, multicolor labeling of DNA synthesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据