期刊
BLOOD
卷 109, 期 4, 页码 1503-1506出版社
AMER SOC HEMATOLOGY
DOI: 10.1182/blood-2006-04-020362
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To generate transgenic mice that express Cre-recombinase exclusively in the megakaryocytic lineage, we modified a mouse bacterial artificial chromosome (BAC) clone by homologous recombination and replaced the first exon of the platelet factor 4 (PM), also called CXCL4, with a codon-improved Cre cDNA. Several strains expressing the transgene were obtained and one strain, 03, was studied in detail. Crossing Q3 mice with the ROSA26-lacZ reporter strain showed that Cre-recombinase activity was confined to megakaryocytes. These results were further verified by crossing the Q3 mice with a strain containing loxP-flanked integrin beta 1. Excision of this conditional allele in megakaryocytes was complete at the DNA level, and platelets were virtually devoid of the integrin beta 1 protein. The Pf4-Cre transgenic strain will be a valuable tool to study megakaryopoiesis, platelet formation, and platelet function.
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