期刊
VIROLOGY
卷 358, 期 2, 页码 436-447出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2006.08.043
关键词
apoptosis; caspase; baculovirus; entomopoxvirus; p35; inhibitor
类别
资金
- NCI NIH HHS [R29 CA078602-05, R29 CA078602, R29 CA78602] Funding Source: Medline
- NCRR NIH HHS [P20 RR107686, P20 RR016475-01, P20 RR16475, P20 RR017686, P20 RR016475, P20 RR017686-057258] Funding Source: Medline
Members of the baculovirus p35 gene family encode proteins that specifically inhibit caspases, cysteine proteases that are involved in apoptosis. To date, p35 homologs have only been found in baculoviruses. We have identified AMVp33, a gene from Amsacta moorei entomopoxvirus with low but significant homology to baculovirus p35 genes. Expression of AMVp33 blocked apoptosis in several different insect and human cell lines. Purified recombinant P33 protein was an efficient inhibitor of insect and human effector caspases, but not initiator caspases. P33 was cleaved by effector caspases, and the resulting cleavage fragments stably associated with the caspases. Mutation of the predicted caspase cleavage site in P33 eliminated cleavage, caspase inhibition and anti-apoptotic function. Thus, AMVp33 encodes a caspase inhibitor similar to baculovirus P35 with a preference for effector caspases. This is the first report of a p35 homolog from any viral or cellular genome outside of the baculovirus family. (c) 2006 Elsevier Inc. All rights reserved.
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