期刊
FEBS LETTERS
卷 581, 期 4, 页码 771-774出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2007.01.047
关键词
aptamer; locked nucleic acid; HIV-1; surface plasmon resonance; 2 '-O-methyl; TAR; tat
资金
- Medical Research Council [MC_U105178803] Funding Source: Medline
- Medical Research Council [MC_U105178803] Funding Source: researchfish
- MRC [MC_U105178803] Funding Source: UKRI
We synthesized and evaluated by surface plasmon resonance 64 LNA/2'-O-methyl sequences corresponding to all possible combinations of such residues in a kissing aptamer loop complementary to the 6-nt loop of the TAR element of HIV-1. Three combinations of LNA/2'-O-methyl nucleoside analogues where one or two LNA units are located on the 3' side of the aptamer loop display an affinity for TAR below 1 nM, i.e. one order of magnitude higher than the parent RNA aptamer. One of these combinations inhibits the TAR-dependent luciferase expression in a cell assay. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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