期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 353, 期 4, 页码 863-868出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2006.12.095
关键词
ethylene responsive element binding protein; cold stress; GCC-box; Solanum tuberosum L.; AP2/ERF domain
To identify components of the plant stress signal transduction cascade and response mechanisms, we screened plant genes using reverse Northern blot analysis, and chose the ethylene responsive element binding protein 1 (StEREBPI) for further characterization. To investigate its biological function in the potato, we performed Northern blot analysis and observed enhanced levels of transcription in response to several environmental stresses including low temperature. In vivo targeting experiments using a green fluorescent protein (GFP) reporter indicated that StEREBPI localized to the nucleus of onion epidermal cells. StEREBPI was found to bind to GCC and DRE/CRT cis-elements and both microarray and RT-PCR analyses indicated that overexpression of StEREBPI induced expression of several GCC box-containing stress response genes. In addition, overexpression of StEREBPI enhanced tolerance to cold and salt stress in transgenic potato plants. The results of this study suggest that StEREBPI is a functional transcription factor that may be involved in abiotic stress responses in plants. (c) 2006 Elsevier Inc. All rights reserved.
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