期刊
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
卷 70, 期 1, 页码 23-29出版社
ELSEVIER
DOI: 10.1016/j.jbbm.2006.11.003
关键词
capillary liquid chromatography; monolithic columns; HILIC mode; oligonucleotides
Hydroxymethyl methacrylate-based monolithic columns for separation of oligonucleotides by capillary liquid chromatography (CLC) were prepared. We optimized composition of the polymerization mixture, which contained the monomer mixture consisting of N-(hydroxymethyl) methacrylamide (HMMAA) and ethylene dimethacrylate (EDMA), and the porogenic system composed of propane-1-ol, butane-1,4-diol and alpha, alpha'-azoisobutyronitrile (AIBN) as initiator. Separations of oligonucleotides were performed in HILIC (hydrophilic-interaction) mode using 100 mM triethylamine acetate (TEAA) in acetonitrile and in water as eluents. The influence of steepness of the mobile phase gradient on separation of the oligonucleotides was evaluated as well as the reproducibility of HMMAA monolith preparation. (c) 2006 Elsevier B.V. All rights reserved.
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